Computational Analysis of Two-dimensional High-throughput Data from Large-scale RNAi Screens and Single-cell Transcriptomics (Q11040)

This publicationprovidesa singularity definition file to reproduce the computational environment along with the scripts to reproduce every figure or table in the revised manuscript using ZetaSuite Perl module and R package. First, generate a new folder and then download all the files into the folder. Then, uncompressed the files DataSets_part1.tar.gz,DataSets_part2.tar.gz,DataSets_part3.tar.gz,DataSets_part4.tar.gz, and scripts.tar.gz. within the folder. Next, move all the files in DataSets_part1 folder,DataSets_part2folder,DataSets_part3folder andDataSets_part4folder to a new folder called DataSets. Finally, run the following scripts to generate thefigures and tables in our manuscript. Regeneration of Figure2 and S2: singularity exec ZetaSuite.sif sh Figure2andS2.sh Regeneration of Figure3 and S3: singularity exec ZetaSuite.sif sh Figure3andS3.sh Regeneration of Figure4 and S4: singularity exec ZetaSuite.sif sh Figure4andS4.sh Regeneration of Figure5 and S5: singularity exec ZetaSuite.sif sh Figure5andS5.sh Regeneration of Figure6 and S6: singularity exec ZetaSuite.sif sh Figure6andS6.sh Regeneration of Figure7 and S7: singularity exec ZetaSuite.sif sh Figure7andS7.sh